Research highlight_simulation_round

Research Highlights: Sea-Ice, Gold Particles and Molecular Imaging

Research from the global scale down to a single DNA base pair, what happens when you follow venom around the body, and rethinking the meaning of labyrinths.


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The Impact of Geophysical Corrections on Sea-Ice Freeboard Retrieved from Satellite Altimetry

Robert Ricker, Stefan Hendricks and Justin F. Beckers

Satellite altimetry is the only method to monitor global changes in sea-ice thickness and volume over decades. Such missions (e.g., ERS, Envisat, ICESat, CryoSat-2) are based on the conversion of freeboard into thickness by assuming hydrostatic equilibrium. Freeboard, the height of the ice above the water level, is therefore a crucial parameter. Freeboard is a relative quantity, computed by subtracting the instantaneous sea surface height from the sea-ice surface elevations. Hence, the impact of geophysical range corrections depends on the performance of the interpolation between subsequent leads to retrieve the sea surface height, and the magnitude of the correction. In this study, we investigate this impact by considering CryoSat-2 sea-ice freeboard retrievals in autumn and spring. Our findings show that major parts of the Arctic are not noticeably affected by the corrections. However, we find areas with very low lead density like the multiyear ice north of Canada, and landfast ice zones, where the impact can be substantial. In March 2015, 7.17% and 2.69% of all valid CryoSat-2 freeboard grid cells are affected by the ocean tides and the inverse barometric correction by more than 1 cm. They represent by far the major contributions among the impacts of the individual corrections.
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On the High Sensitivity of the Electronic States of 1 nm Gold Particles to Pretreatments and Modifiers

Oxana Martynyuk, Yulia Kotolevich, Rodrigo Vélez, Jesus Efren Cabrera Ortega, Hugo Tiznado, Trino Zepeda Partida, Josué D. Mota-Morales, Alexey Pestryakov and Nina Bogdanchikova

In this paper, the effect of modifiers and pretreatments on the electronic states of 1 nm gold nanoparticles (AuNPs) supported on silica was systematically studied. AuNPs deposited on silica (particle size of 2–4 nm) modified with Ce, La and Fe oxides, were studied by FTIR of adsorbed CO after different redox treatments at 100, 300 and 500 °C. This study was conducted at room temperature to allow detecting the electronic states of gold, which is more likely involved in CO oxidation at the same temperature. AuNP size distribution was measured by HRTEM. It is shown that the electronic state of gold species (Aunδ−, Au0, Aunδ+, Au+) in 1 nm AuNPs is sensitive to the modifier as well as to the temperatures of redox pretreatments. Supports modified with the same additives but containing larger AuNPs (~3, 4, 5, and 7 nm) were also studied. They showed that Au0 remains stable irrespective of additives and redox pretreatments, indicating no significant effect of such treatments on the electronic properties of larger AuNPs. Samples with a predominant AuNP size of 2 nm are an intermediate case between these two groups of materials.

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6-Pyrazolylpurine as an Artificial Nucleobase for Metal-Mediated Base Pairing in DNA Duplexes

J. Christian Léon, Indranil Sinha and Jens Müller

The artificial nucleobase 6-pyrazol-1-yl-purine (6PP) has been investigated with respect to its usability in metal-mediated base pairing. As was shown by temperature-dependent UV spectroscopy, 6PP may form weakly stabilizing 6PP–Ag(I)–6PP homo base pairs. Interestingly, 6PP can be used to selectively recognize a complementary pyrimidine nucleobase. The addition of Ag(I) to a DNA duplex comprising a central 6PP:C mispair (C = cytosine) leads to a slight destabilization of the duplex. In contrast, a stabilizing 6PP–Ag(I)–T base pair is formed with a complementary thymine (T) residue. It is interesting to note that 6PP is capable of differentiating between the pyrimidine moieties despite the fact that it is not as sterically crowded as 6-(3,5-dimethylpyrazol-1-yl)purine, an artificial nucleobase that had previously been suggested for the recognition of nucleic acid sequences via the formation of a metal-mediated base pair. Hence, the additional methyl groups of 6-(3,5-dimethylpyrazol-1-yl)purine may not be required for the specific recognition of the complementary nucleobase.
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Magnetophoretic Sorting of Single Cell-Containing Microdroplets

Younggeun Jo, Fengshan Shen, Young Ki Hahn, Ji-Ho Park and Je-Kyun Park

Droplet microfluidics is a promising tool for single-cell analysis since single cell can be comparted inside a tiny volume. However, droplet encapsulation of single cells still remains a challenging issue due to the low ratio of droplets containing single cells. Here, we introduce a simple and robust single cell sorting platform based on a magnetophoretic method using monodisperse magnetic nanoparticles (MNPs) and droplet microfluidics with >94% purity. There is an approximately equal amount of MNPs in the same-sized droplet, which has the same magnetic force under the magnetic field. However, the droplets containing single cells have a reduced number of MNPs, as much as the volume of the cell inside the droplet, resulting in a low magnetic force. Based on this simple principle, this platform enables the separation of single cell-encapsulated droplets from the droplets with no cells. Additionally, this device uses only a permanent magnet without any complex additional apparatus; hence, this new platform can be integrated into a single cell analysis system considering its effectiveness and convenience.
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Biodistribution and Lymphatic Tracking of the Main Neurotoxin of Micrurus fulvius Venom by Molecular Imaging

Irene Vergara, Erick Y. Castillo, Mario E. Romero-Piña, Itzel Torres-Viquez, Dayanira Paniagua, Leslie V. Boyer, Alejandro Alagón and Luis Alberto Medina

The venom of the Eastern coral snake Micrurus fulvius can cause respiratory paralysis in the bitten patient, which is attributable to β-neurotoxins (β-NTx). The aim of this work was to study the biodistribution and lymphatic tracking by molecular imaging of the main β-NTx of M. fulvius venom. β-NTx was bioconjugated with the chelator diethylenetriaminepenta-acetic acid (DTPA) and radiolabeled with the radionuclide Gallium-67. Radiolabeling efficiency was 60%–78%; radiochemical purity ≥92%; and stability at 48 h ≥ 85%. The median lethal dose (LD50) and PLA2 activity of bioconjugated β-NTx decreased 3 and 2.5 times, respectively, in comparison with native β-NTx. The immune recognition by polyclonal antibodies decreased 10 times. Biodistribution of β-NTx-DTPA-67Ga in rats showed increased uptake in popliteal, lumbar nodes and kidneys that was not observed with 67Ga-free. Accumulation in organs at 24 h was less than 1%, except for kidneys, where the average was 3.7%. The inoculation site works as a depot, since 10% of the initial dose of β-NTx-DTPA-67Ga remains there for up to 48 h. This work clearly demonstrates the lymphatic system participation in the biodistribution of β-NTx-DTPA-67Ga. Our approach could be applied to analyze the role of the lymphatic system in snakebite for a better understanding of envenoming.

 

…and for something with a different perspective
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Becoming the Labyrinth: Negotiating Magical Space and Identity in Puella Magi Madoka Magica

Sara Cleto  and Erin Kathleen Bahl

In the magical girl anime series Puella Magi Madoka Magica, middle-school girls receive the power and responsibility to fight witches in exchange for making a wish. The series has connections to many different genres and narrative traditions within the realm of folkloristics. However, the folkloric genre most relevant to the ethos and aesthetics of Madoka is that of the fairy tale. Drawing on Bill Ellis’s concept of “fairy-telling” and scholarship on new media composition, in this paper we seek to investigate labyrinths as acts of embodied composing—not lairs of evil or destruction but rather creative material memory work that negotiates grief and despair. Many of the series’ action sequences unfold in “labyrinths,” the magical spaces controlled by witches. By composing a labyrinth, witches can simultaneously reshape their environment and create a powerful statement about identity through personalized performance in narrative spaces that they control. In particular, we argue that both the frameworks of “fairy tale” and “new media” give us useful analytical resources for beginning to make sense of the intricately complex phenomenon ofMadoka’s labyrinths.

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